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Methods for estimating divergence times from molecular data have improved dramatically over the past decade, yet there are few studies examining alternative taxon sampling effects on node age estimates. Here, I investigate the effect of undersampling species diversity on node ages of the South American lizard clade Liolaemini using several alternative subsampling strategies for both time calibrations and taxa numbers.

Penalized likelihood PL and Bayesian molecular dating analyses were conducted on a densely sampled taxa mtDNA-based phylogenetic hypothesis of Iguanidae, including 92 Liolaemini species. Using all calibrations and penalized likelihood, clades with very low taxon sampling had node age estimates younger than clades with more complete taxon sampling. The effect of Bayesian and PL methods differed when either one or two calibrations only were used with dense taxon sampling.

Bayesian node ages were always older when fewer calibrations were used, whereas PL node ages were always younger. This work reinforces two important points: 1 whenever possible, authors should strongly consider adding as many taxa as possible, including numerous outgroups, prior to node age estimation to avoid considerable node age underestimation and 2 using more, critically assessed, and accurate fossil calibrations should yield improved divergence time estimates.

Alternative taxon sampling strategies are known to affect many facets of phylogenetic reconstruction [ 1 — 3 ]. Linder et al. This study found that mean estimated ages of focal nodes were significantly younger with sparser taxon sampling than when taxa from the complete dataset were included. In addition, the more distant the focal node was from the calibration point, the more sensitive estimation effects were on nodal ages if the taxa were undersampled, especially for nonparametric rate-smoothing NPRS methods; but penalized likelihood PL and Bayesian methods also were prone to these effects especially if undersampling was large.

Subsequent studies following up on these important results are lacking in the literature. This is especially important because numerous multigene studies estimate divergence times but often significantly undersample large clades using a single representative species in place of higher taxonomic groups.

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A “best of” list of 10 kid-friendly Minecraft servers and tips for parents on how to spot Minecraft servers for kids and families.

Playing Minecraft on a public server can take the game to a whole new level. In addition, most servers feature huge, prebuilt worlds, with amazing cities and buildings, transport networks, and mini-games for your kid to explore and enjoy. If they go on a server, how will I know they will be safe from bad language, bullying, or online predators? Joining a public Minecraft server is very easy. Just open the Minecraft launcher and click Play to run the main Minecraft game.

Once you reach the main title screen, you click the Multiplayer button, then click the Add Server button to add the server. Now type a name for the server in the Server Name box, then type the server address in the Server Address box. Typically this will be a domain name, such as mc. Then click Done to add the server to your server list:. To add a server, click Add Server and enter the server details.

You can then connect to the server with the Join Server button.

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